Journal: Virology Journal

Article Title: Inhibitor analysis revealed that clathrin-mediated endocytosis is involed in cellular entry of type III grass carp reovirus

doi: 10.1186/s12985-018-0993-8

Figure Lengend Snippet: Variables from the questionnaire of the HBSC study analyzed in this study

Article Snippet: Inhibitors were prepared as follows: pistop2, dynasore, rottlerin, nystatin, wortmannin, bafilomycin A1, Latrunculin B, nocodazole, IPA-3, and amiloride were purchased from ApexBio (Houston, USA).

Techniques: Concentration Assay, Inhibition

Journal: Virology Journal

Article Title: Inhibitor analysis revealed that clathrin-mediated endocytosis is involed in cellular entry of type III grass carp reovirus

doi: 10.1186/s12985-018-0993-8

Figure Lengend Snippet: Effect of inhibitors on the production of progeny virus. CIK cells were treated with different inhibitors at the indicated concentrations and then infected with GCRV104 (MOI = 5) for 5 days. Uninternalized virions were removed at 1 hpi. Rt-PCR assay of virus yield in the supernatants. a Pistop2 (5 μM and 1 μM) and CPZ (10 μM, 5 μM, and 1 mM) inhibit GCRV104 infection. b Rottlerin (5 μM and 2 μM) and wortmannin (5 μM and 2 μM) inhibit GCRV104 infection. c and d Nystatin (15 μM, 3 μM), Methyl-β-cyclodextrin (1 mM, 0.5 mM, 0.1 mM), Latrunvulin B (0.5 μM, 0.25 μM, 0.05 μM), nocodazole (10 μM, 5 μM, 1 μM), IPA-3 (10 μM, 5 μM, 1 μM), Amiloride (10 μM, 5 μM, 1 μM), and Bafilomycin A1 (2 nM, 1 nM, 0.2 nM) were used for analysis different pathways. Asterisks represent a significant difference from the control (unpaired t-test, * P < 0.05 and ** P < 0.01)

Article Snippet: Inhibitors were prepared as follows: pistop2, dynasore, rottlerin, nystatin, wortmannin, bafilomycin A1, Latrunculin B, nocodazole, IPA-3, and amiloride were purchased from ApexBio (Houston, USA).

Techniques: Virus, Infection, Reverse Transcription Polymerase Chain Reaction, Control